Abstract

Developing precise bioconjugation methods is essential for the progress of biotechnology and biomedical devices. For biomolecule immobilization, chemical reactions with high specificity, efficiency and biocompatibility are desired. If defined immobilization of multiple biomolecules on the same surface is needed, the individual bioconjugation reactions need to be orthogonal to each other.

Chemical vapor deposition (CVD) polymerization is a versatile coating process that effectively decouples the surface chemistry from the bulk composition. The CVD polymerization of functionalized [2.2]paracyclophanes can result in functionalized poly(p-xylylene) coatings with a wide range of different groups including alkynes, active esters, aldehydes, ketones, or anhydrides.

This powerful platform to functionalize virtually any solid surface also allows us to apply different functional groups on the same surface by copolymerization or surface micro-engineering. We explored 3 different combinations of bio-orthogonal reactions for biomolecule immobilizations on 3 different multifunctional polymer coatings.