Sensitivity improvement in capillary electrophoresis using organo-auqeous separation buffers and thermal lens detection
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Autor:
Bendrysheva,. S.N. / Proskunin, M.A./ Pyell, U. / Faubel W. (2006)
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Quelle:
Anal. Bioanal. Chem 385 (2006), 1492-1503
- Datum: 2006
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Bendrysheva,. S.N. / Proskunin, M.A./ Pyell, U. / Faubel W. (2006): „Sensitivity improvement in capillary electrophoresis using organo-auqeous separation buffers and thermal lens detection“. In: Anal. Bioanal. Chem 385 (2006), 1492-1503
Abstract
It is shown that organo-aqueous separation buffers show much promise when used in capillary electrophoresis separations with photothermal (thermal lens) detection systems. Acetonitrile–water and methanol–water mixtures were selected, as conventionally used in capillary electrophoresis.
It is shown that, despite more sophisticated experimental conditions (significant heat outflow from the capillary body) and peak detection, the theoretical ratio of the thermal lens signal for a binary mixture to the thermal lens signal for an aqueous solution (or the corresponding ratio obtained experimentally under bulk batch conditions) can be used to predict the sensitivity of thermal lens detection in capillary electrophoresis.
The limits of detection for 2-, 3-, and 4-nitrophenols selected as model compounds in 70% v/v acetonitrile separation buffers are 1×10−6 M, 1×10−6 M and 3×10−7 M, respectively, and are therefore decreased by a factor of six compared to thermal lens detection in aqueous separation buffers. The overall increase in the thermal lens detection sensitivity in a 100% ACN buffer is a factor of 13.
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It is shown that, despite more sophisticated experimental conditions (significant heat outflow from the capillary body) and peak detection, the theoretical ratio of the thermal lens signal for a binary mixture to the thermal lens signal for an aqueous solution (or the corresponding ratio obtained experimentally under bulk batch conditions) can be used to predict the sensitivity of thermal lens detection in capillary electrophoresis.
The limits of detection for 2-, 3-, and 4-nitrophenols selected as model compounds in 70% v/v acetonitrile separation buffers are 1×10−6 M, 1×10−6 M and 3×10−7 M, respectively, and are therefore decreased by a factor of six compared to thermal lens detection in aqueous separation buffers. The overall increase in the thermal lens detection sensitivity in a 100% ACN buffer is a factor of 13.
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