Simplified Purification of Equine Chorionic Gonadotropin (eCG) - An Example of the Use of Magnetic Microsorbents for the Isolation of Glycoproteins from Serum

  • Autor: Müller, C. / Wagner, K. / Frankenfeld, K. / Franzreb, M. (2011)

  • Quelle:

    Biotechnology Letters 33 (2011), 5, 929–936
  • Datum: 2011

  • Müller, C. / Wagner, K. / Frankenfeld, K. / Franzreb, M. (2011): „Simplified Purification of Equine Chorionic Gonadotropin (eCG) - An Example of the Use of Magnetic Microsorbents for the Isolation of Glycoproteins from Serum“. In: Biotechnology Letters 33 (2011), 5, 929–936

Abstract

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Classical purification of the glycoprotein equine chorionic gonadotropin (eCG) from serum includes pH fractionation with metaphosphoric acid, two ethanol precipitation steps as well as dialysis followed by fixed-bed chromatography. A simplified process requiring only 1/3 of the solvent and improving the yield from 53 to 65% has been developed.

The process comprises an ultra-/diafiltration step after the first ethanol precipitation, directly followed by an adsorption/desorption procedure based on magnetic microadsorbents with N,N-diethyl-ammonium functionalization. The process reaches an overall purification factor of eCG of more than 1800 and an average product activity of 1300 IU(ELISA)/mg. After adapting the parameters of the fractionation and the type of magnetic microadsorbents, the new concept is likely to be transferable to other serum proteins.