Digital In-line Holography with femtosecond VUV radiation provided by the free-electron laser FLASH

  • chair:

    Rosenhahn, A. / Staier, F. / Nisius, T. / Schäfer, D. / Barth, R. / Christophis, C. / Stadler, L. / Streit-Nierobisch, S. / Gutt, C. / Mancuso, A. / Schropp, A. / Gulden, J. / Reime, B. / Feldhaus, J. / Weckert, E. /  Pfau, B. / Günther, C. / Könnecke, R. / Eisebitt, S. / Martins, M. / Faatz, B. / Guerassimova, N. / Honkavaara, K. / Treusch, R. / Saldin, E. / Schreiber, S. / Schneidmiller, E. / Yurkov, M. / Vartaniants, I. / Grübel, G. / Grunze, M. / Wilhein, T. (2009)
  • place:

    Optics Express 17 (2009), 10, 8220
  • Date: 2009

  • Rosenhahn, A. / Staier, F. / Nisius, T. / Schäfer, D. / Barth, R. / Christophis, C. / Stadler, L. / Streit-Nierobisch, S. / Gutt, C. / Mancuso, A. / Schropp, A. / Gulden, J. / Reime, B. / Feldhaus, J. / Weckert, E. / Pfau, B. / Günther, C. / Könnecke, R. / Eisebitt, S. / Martins, M. / Faatz, B. / Guerassimova, N. / Honkavaara, K. / Treusch, R. / Saldin, E. / Schreiber, S. / Schneidmiller, E. / Yurkov, M. / Vartaniants, I. / Grübel, G. / Grunze, M. / Wilhein, T. (2009): „Digital In-line Holography with femtosecond VUV radiation provided by the free-electron laser FLASH“. In: Optics Express 17 (2009), 10, 8220

Abstract

Femtosecond vacuum ultraviolet (VUV) radiation provided by the free-electron laser FLASH was used for digital in-line holographic microscopy and applied to image particles, diatoms and critical point dried fibroblast cells. To realize the classical in-line Gabor geometry, a 1 mu m pinhole was used as spatial filter to generate a divergent light cone with excellent pointing stability. At a fundamental wavelength of 8 nm test objects such as particles and diatoms were imaged at a spatial resolution of 620 nm. In order to demonstrate the applicability to biologically relevant systems, critical point dried rat embryonic fibroblast cells were for the first time imaged with free-electron laser radiation.

 

 

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