Etablierung von enzymatischen Mehrkomponentenreaktionen für die kontinuierliche Synthese in einem Enzym-Membran-Reaktor

Background:
On the way to sustainable production of complex chemical compounds, enzymes as highly selective catalysts active under mild conditions enable the use of more efficient and environmentally friendly reaction pathways.
To achieve high conversion rates and high space-time yields in such processes, enzymes can be immobilised and used in flow reactors in a second step. Among the different types of flow reactors, enzyme membrane reactors (EMR) offer the possibility to retain immobilisate as well as substrate and product by choosing suitable membranes, while inhibiting by-products are removed in-situ. In this work, multi-component reactions catalysed by a lipase from the literature are to be established in a "batch process" (Eppendorf vessels) and, in a second step, continuously implemented in a flow reactor.

Task:
Within the scope of the bachelor thesis, multicomponent reactions are to be established for application in a continuous EMR. The work packages include:

- Selection of suitable reactions from the literature
- Synthesis and purification of product standards for the establishment of rapid analytics
- Establishment of quantitative analytics using HPLC, GC-MS or ESI-MS/MS

In case of a master thesis additionally:

- Modelling and prediction of reactor behaviour using COMSOL
- Continuous synthesis in the flow reactor

Supervision:
In addition to the scientific supervision by Prof. Dr.-Ing. Matthias Franzreb, Mr./Mrs. xx is supported in the practical implementation of the work by Mr. M. Sc. Ulrich Thiele.  

Contact:
Ulrich.thiele@kit.edu